Lee, H.-L., Lin, C.-S., Kao, S.-H. & Chou, M.-C. Gallic acid induces G1 phase arrest and apoptosis of triple-negative breast cancer cell MDA-MB-231 via p38 mitogen-activated protein kinase/p21/p27 axis. Deletion of this gene has been shown to induce a complete loss of GlcNAc expression accompanied by the progressive development of Differentiated-type gastric adenocarcinoma in a hyperplasia-dysplasia-carcinoma continuum that is strictly confined at the pyloric mucosa of the resulting mutant animals28. J. HerbMed Pharmacol. 7 Health Benefits of Bee Propolis. Effects of local administration of propolis and its polyphenolic compounds on tumor formation and growth. *P<0.05 using Independent sample t-test. As illustrated in Fig. Scientific Reports (Sci Rep) J. Evid. Abstract Propolis is a dark-colored bee product derived from plant resins and has many health benefits. What Are The Benefits Of Bee Propolis Cancer Lett. 68, 394424, https://doi.org/10.3322/caac.21492 (2018). Therefore, the deliberate application of EEP under the circumstance of already existing tumor as in the case of A4gnt KO mice in this present work proffered an indisputable justification for its pronounced therapeutic anti-cancer property. Oncol. J. Med. They are greatly valued as an effective pollinator and are mostly preferred for propolis production due to higher cost efficiency and increased resistance to parasitic infestation39. 100, 114117, https://doi.org/10.1016/j.jep.2005.05.004 (2005). Biol. Animals were injected intraperitoneally with Bromodeoxyuridine solution (BrdU; 10mg/kg) an hour before sacrifice to allow labeling of rapidly proliferating S-phase cells28. M.J.D., S.K. Means with different letter are significant at P<0.05 using ANOVA with Tukey-HSD posttest. Tang, H. et al. CAS Google Scholar. Some in vivo tests have Stingless Bee Propolis: New Insights for Anticancer Drugs WebAny thoughts? Bras. J. Ethnopharmacol. P17005H03). Cell cycle progression is a well-orchestrated process requiring tight coordination of the homeostatic balance between cyclin/cyclin-dependent kinase protein (CDK) complexes and their inhibitor proteins (CKI)52. 1. Naturwissenschaften 98, 329337, https://doi.org/10.1007/s00114-011-0770-7 (2011). Article 9, 336, https://doi.org/10.3389/fphar.2018.00336 (2018). Thrumurthy, S. G., Chaudry, M. A., Chau, I. Propolis from the stingless bee Trigona encisa from East Kalimantan, Indonesia, induces in vitro cytotoxicity and apoptosis in cancer. M.A.E. Another thing worth mentioning also was the observation that no glaring difference could be deciphered between the distilled water and EEP-treated wildtype C57BL/6J animals with respect to their gross morphology (Fig. Therefore, on the basis of all these findings, it can be deduced that EEP may exert promising in vivo anti-tumor efficacy against differentiated-type gastric adenocarcinoma. Hunter, T. & Pines, J. Cyclins and cancer ii: Cyclin D and CDK inhibitors come of age. Kalogeropoulos, N., Konteles, S.J., Troullidou, E., Mourtzinos, I. Honey and propolis abrogate neurologic deficit and neuronal damage in the hippocampus and cerebral cortex of ischemic stroke rats. IJARNP 10, 715 (2017). Evan, G. I. and K.U. 78, 302310, https://doi.org/10.1159/000321703 (2011). 2A), gastric mucosal thickness (Fig. Prod. I tested positive for COVID 3 weeks ago and have had a lingering sore throat and cough that would not go away. J. Ethnopharmacol. The number of gastric cancer cells undergoing apoptosis, expressed as % apoptotic cells, was estimated by counting the number of FITC-dUTP-labeled cells per 1000 cells and the average measurement from three independent counts was obtained. Sulaiman, G. M. et al. & Locsin, A. J. Morphol. 338, 1116, https://doi.org/10.1016/S0009-8981(03)00323-1 (2003). The oven program, which started with an initial temperature of 100C for 4minutes, was heated up to 320C at a rate of 10C /min, and held for 11min. Carcinog. Cancer. All analyses were performed using SPSS v.23 (IBM Corp., Armonk, NY, USA) and values with P<0.05 were considered statistically significant. Catchpole, O., Mitchell, K., Bloor, S., Davis, P. & Suddes, A. Antiproliferative activity of New Zealand propolis and phenolic compounds vs human colorectal adenocarcinoma cells. Propolis, a bee-derived substance consisting of bees own salivary secretion together with collected resins, exudates, and oils, is a natural healing agent that has been highly regarded owing to its low toxicity, relative safety, and pronounced biological functionality13. Med. Those resins have Treatment with gallic acid and pterostilbene has been earlier shown to elicit significant growth restriction of MDA-MB-231 triple-negative breast adenocarcinoma and AGS gastric carcinoma72,73. & Allum, W. Does surgery have a role in managing incurable gastric cancer? For centuries, people believed that propolis was used by bees to plug holes in the hive. Chem. Since few chemical-profiling studies by LC-MS analysis have already been conducted on samples of Philippine stingless bee propolis including those obtained from the same colonies as in the present work41,42, we decided to utilize a different analytical platform, hence employing GC-MS/MS analysis. PubMed Central Eur. Use of Stingless Bee Propolis and Geopropolis against CancerA Lastly, we aimed to validate the role of apoptosis by analyzing DNA fragmentation through TUNEL Assay. et al. bee propolis for preventing and healing non-communicable diseases Parul Dube February 25, 2022 Getty Image/ Shutterstock Propolis is a waxy or resinous mixture made by honeybees. First Online: 25 September 2021 540 Accesses Part of the Food Bioactive Ingredients book series (FBC) Abstract Caffeic acid phenethyl ester (CAPE) is the vital natural phenolic chemical composite present in a variety of plants which is also abundantly present in the honeybee hives as propolis. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Nat. Additionally, propolis from the stingless bees Tetragonula pagdeni, Lepidotrigona ventralis, and Lepidotrigona terminata induced death in four human cancer Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines. Sci. Nevertheless, irrespective of the type, they are virtually comparable vis--vis the extent of their biofunctional activities such as anticancer, antimicrobial, anti-inflammatory, antioxidant, and wound healing properties15,36,37,38. Analysis was run in duplicates. Laboratory studies suggest that the compounds in propolis may inhibit cancer cell growth or induce cell death in cancer cells. Chemoradiotherapy after surgery compared with surgery alone for adenocarcinoma of the stomach or gastroesophageal junction. Chim. Farmacogn. Scientist 94, 1422 (2011). Propolis is a sticky substance gathered by the bees from the leaf buds and bark of trees. Drug Discov. Prior to administration, EEP was diluted to distilled water thereby bringing the vehicle ethanol to a negligible concentration. Scale bar: 100m (D) Comparison of the number of cells undergoing apoptosis expressed as % apoptotic cells between untreated and EEP-treated groups in three human GC cell lines. Upon sacrifice, careful scrutiny of the harvested mouse stomach tissues revealed a significant regression of the gross mucosal elevation in the A4gnt KO+EEP treatment group in comparison to the untreated control group (Fig. WebWe have earlier investigated the molecular mechanism of anticancer activity in CAPE and reported that it activates DNA damage signaling in cancer cells. (F) Comparison of the number of CD3-positive T-lymphocytes among the different treatment groups expressed as mean count per 100m area taken from at least three different points with the highest cell density. This was performed in three independent experiments. Int. Pereira-Filho, R. N. et al. On the other hand, gallic acid, protocatechuic acid, and pterostilbene were some of the identified phenolic acids. He, G. et al. J. Agric. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in As shown in Fig. J. Evid. Last updated on Aug 8, 2022. Cell 14, 159169, https://doi.org/10.1016/j.devcel.2008.01.013 (2008). However, propolis treatment in all these above-mentioned studies, in converse to our study, has been carried out either prophylactically or concurrently with tumor induction. Laboratory of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, Mark Joseph Desamero,Shigeru Kakuta,Yulan Tang&Shigeru Kyuwa, Department of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Baos, Laguna, 4031, Philippines, Mark Joseph Desamero&Maria Amelita Estacio, UPLB Bee Program, University of the Philippines Los Baos, Laguna, 4031, Philippines, Mark Joseph Desamero,Maria Amelita Estacio&Cleofas Cervancia, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baos, Laguna, 4031, Philippines, Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, James Kenn Chambers,Kazuyuki Uchida&Hiroyuki Nakayama, Laboratory of Marine Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, Department of Molecular Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 3908621, Japan, You can also search for this author in (B) Cell cycle analysis of AGS cells using propidium iodide staining depicting the representative raw data of the untreated control and EEP-treated cells (IC50 value @ 48h) (left) and graphical analysis of the cell cycle distribution in the sub-G1, G0/G1, S, G2/M, and multi-nuclear phases (right). WebOverall rating 5.0. 3, Supplementary Table3). 113, 158164, https://doi.org/10.1016/j.jaap.2014.12.006 (2014). J.K.C., K.U. Stingless Bee Propolis: New Insights for Anticancer Drugs 357, 18101820 (2007). DNA nick-end labeled cells were finally mounted in a mounting medium with DAPI (Vectashield, Vector Laboratories, Inc., Burlingame, CA, USA) and visualized under a confocal laser-scanning microscope (LSM510 Version 2.02; Carl Zeiss, Jena, Germany). CAS Therefore, in light of this obvious indication for alternative mechanistic pathway, and in conjunction with the present in vitro data, we examined thereafter those genes associated with cell cycle progression and apoptosis. However, according to the latest GLOBOCAN 2018 estimates, it continues to be a major disease burden affecting approximately 783,000 individuals whose gravity has been widely observed in East Asian countries like Japan, Mongolia, and Korea1. WebPropolis has shown efficacy against brain, head and neck, skin, breast, liver, pancreas, kidney, bladder, prostate, colon and blood cancers. CAS However, one caveat of this finding was that the enhanced anti-proliferative activity might be due to cell line growth capacity17. Rev. Clin. Article ID 280120, 11 pages, https://doi.org/10.1155/2014/280120 (2014). Pharmacol. All spectra were recorded in the mass range of 45600m/z. In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis. Dual effect of Algerian propolis on lung cancer: Anti-tumor and chemoproventive effects involving antioxidant activity. **P<0.05 using Kruskal-Wallis test. Bee Healthy: How Propolis Can Help Savannah Bee Company Int. Front. Anticancer Activity of Propolis and Its Compounds - PMC
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