aqueous solutions containing radioactive material and chloroform that occur in solutions generated by the neutralization of radioactive trichloroacetic acid solutions, certain gel electrophoresis waste (e.g., methanol or acetic acid containing radionuclides), and. Validation process shall be done as It is essential that you understand how to operate microbial culture shall be discarded in the incineration pit. Note that recycling and reclamation extend to reclamation of energy as well as materials. Annexure I : Disposal of Waste, Copyright 2023 Pharmaceutical Guidelines, SOP On Disposal of Waste Generated in Quality Control Lab, List of ICH Quality Guidelines for Pharmaceutical Industry, SOP List For Pharmaceutical Quality Assurance, SOP on Handling of Incidents and Deviations. Under federal, state, and local law, there are various exemptions, exclusions, effluent limits, and permitting requirements that may apply to laboratory wastewaters. Note that these are regulated under this listing only if they have been used (spent). Hazard reduction is part of the broad theme of pollution prevention that is encouraged throughout this book. Most institutions designate a room or facility equipped with good ventilation, effluent trapping, and fire suppression to contain and manage on-site. Note that unemptied source containers of some drugs are EPA-listed hazardous waste and must be managed as a regulated chemical waste. Performing a hazard reduction procedure as part of an experiment has considerable economic advantages by eliminating the necessity to accumulate, handle, store, transport, and treat hazardous waste after the experiment. This is a special concern with commingled waste solvents, which must be chemically compatible to ensure that heat generation, gas evolution, or another reaction does not occur. Using labpacks is quite simple. Procedures presented in this book are intended to be carried out only by, or under the direct supervision of, a trained scientist or technologist who understands the chemistry and hazards involved. Rigorous application of waste minimization principles can often solve the problems of managing mixed waste. More details can be found in section 8.B.6, below. Chemical waste, which can again be divided into many categories: Infectious waste must be incinerated. PDF Standard Operating Procedure Management of Laboratory Waste EPA numbers must be provided for each line item on the manifest. 7.1.1 All potential waste streams that arise from laboratory operations needs to be assessed and an appropriate. used flammable (e.g., toluene) liquid scintillation cocktails. 2. Radioactive needles contaminated with infectious agents or blood should be autoclaved as described above, and then incinerated on-site or shipped to a LLRW site. Make the facilities for implementing the Waste Segregation and Disposal SOP. Clearly, the best approach to laboratory waste is preventing its generation. However, treatment of chemical hazardous waste has regulatory implications that must be considered. Proper care shall be taken while making slurry and disposal. 2. Properly cleaning containers as described above, and recycling or disposing of them with the normal trash, reduces costs as well as the volume of hazardous waste generated. An explanation of the federal allowance to treat waste in an accumulation container has been published in the Federal Register (1986). Store waste in clearly labeled containers in a designated location that does not interfere with normal laboratory operations. There are various methods for physical and chemical treatment of hazardous wastes, as well as methods for recycling, reclamation, and recovery of valuable materials contained in the waste. For example, vapors or aerosols from a reaction, distillation, or evaporation must not lead to escape of unsafe levels of radioactive materials into the atmosphere. To test for hydroperoxides in water-insoluble organic solvents, dip the starch-iodine test paper into the solvent, and let it dry. (If animal or commercial incineration is unavailable, methods in section 8.C.3.3, below, may be adaptable to chemicalbiological waste.). Incineration of mercury and other toxic metals is restricted; recycling, recovery, or encapsulation is environmentally preferred. A container or inner liner of a container that contained hazardous waste is empty under federal regulations if all waste has been removed by standard practice and no more than 2.5 cm (1 in.) If the sample is water-soluble, test the pH of a 10% aqueous solution. All chemicals must be characterized sufficiently for safe transportation off-site. a radioactive waste. If it is an insoluble liquid, note whether it is less or more dense than water (i.e., does it float or sink?). This SOP covers procedures for the disposal of waste from human or animal healthcare and or related research according to Chapter 18 of the EWC categorization and includes; procedures for the treatment of Contaminated labware may include cultures, stocks, petri plates, and other disposable laboratory items (e.g., gloves, pipettes, and tips). The radioactive still bottom, containing 1 to 5% methanol and acetic acid, can usually be neutralized, diluted, and disposed of in the sanitary sewer. o For plant tissue cleanup, use 10% bleach or 70% ethanol. ), Use an appropriate container for the collection of liquid waste. (See Chapter 4, section 4.D.3.2, and Chapter 6, section 6.G.3, for information on the hazards of peroxides.). The container shall be properly sealed and labeled as Disposal waste from QC. preparation and execution of this SOP. FIGURE 8.2 Example of Uniform Hazardous Waste Manifest. Make provision for spill control in case of an accident during transportation and handling. hbbd```b``"jV Examples of laboratory mixed waste include. Presence of peroxides. The removal efficiency for a number of volatile and semi-volatile compounds is in excess of 99.99%. While the expense and practicality of these technologies is largely based on the specific nature and volume of the material, treatment or recycling is preferable to incineration for some hazardous wastes. SOP On Disposal of Waste Generated in Quality Control Lab Medical waste incineration is also available through commercial waste haulers. Chemical decontamination (e.g., soaking in bleach) may be appropriate if it can be done without risking personal exposure, increasing waste volumes, or creating a waste that is difficult to handle (e.g., wet waste). In some cases, waste is stabilized or encapsulated to enable safe storage and transport. are disposed in the scrap yard after shredding or defacing. To instruct users in the safe disposal of waste from the lab and its proper removal . The appropriate TSDF should be selected by the generator, although often this decision is heavily influenced by the waste disposal firm, which usually has ties to selected TSDFs or will make recommendations. The generator must obtain assurance, in terms of documentation, permits, records, insurance and liability coverage, and regulatory compliance history, that the chosen service provider is reliable. tion. preparation and execution of this SOP. Waste management options include recycling, laboratory methods, management at institutional waste facilities, and treatment and disposal at commercial sites. The procedures involve decontaminating the mixed hazardous chemical and biohazardous waste to eliminate the biohazardous characteristics of the waste prior to disposal. Such procedures, as part of an academic or industrial experiment, usually involve small amounts of materials which can be handled easily and safely by laboratory personnel. The other categories of listed waste common to laboratories are the U and P lists, which include many chemicals frequently found in laboratories. Non-hazardous laboratory waste Laboratory recyclables Glass from laboratories Hazardous laboratory waste Hazardous wastes are listed below and must be disposed of in accordance with the relevant procedures. Comments having links would not be published. In some cases the disposal method and ultimate fate of the waste require that different wastes not be accumulated together. Princeton, NJ 08540[emailprotected]609-258-5294, 2023 The Trustees of Princeton University, Hazard Identification and Risk Assessment, Laboratory Access and Training Recommendations, Laboratory Equipment and Engineering Controls, SHIELD - Safety, Health, Inspection and Equipment Logistics Database, Administration of Biological and Chemical Hazards to Animals, Medical Clearance and Safety Training For Animal Researchers, Recombinant and Synthetic Nucleic Acid Molecules, Shipping and Receiving Biological Material, X-Ray Machines & Other Radiation-Producing Equipment, Personal Protective Equipment (PPE) for Construction, Staff Achievements and Departmental Honors, Authorization to Use Radioactive Materials, Lab Waste Collection Procedures - Solid.pdf, Waste Log for Long-Lived & Liq Scint Pails.pdf. This strategy could result in reducing or eliminating one hazardous constituent from the waste stream and managing the waste as a single-hazard waste. Several commercial disposal facilities that accept mixed waste from off-site generators do exist in the United States. The TCLP test is primarily for solid materials; liquids are typically evaluated on a straight concentration basis. This rule promotes the use of standard microbiological practices including safe practices for handling biohazardous wastes. As stated in the introduction to this chapter, begin no activity unless a plan for disposal of all waste, hazardous and nonhazardous, has been formulated. Alternatively, addition of 1 mL of a freshly prepared 10% solution of potassium iodide to 10 mL of an organic liquid in a 25-mL glass cylinder produces a yellow color if peroxides are present. As with all putrescible waste, waste should be refrigerated, frozen, or otherwise preserved during accumulation, transport, and storage. See section 8.B.3.4, above, for information on rinsing and disposal of empty containers. Purchase chemicals and radioactive materials in quantities necessary for a planned experiment to avoid creating surplus materials that may end up as waste. 2.0 SCOPE : These numbers are from the hazardous waste lists and characteristics associated with each waste. For example, because nonhalogenated solvents are more suitable for fuel blending, many laboratories collect halogenated and nonhalogenated solvent wastes separately. Records of costs, internal tracking, and so forth can provide information on the success of the hazardous waste management program. Standard Operating Procedure- Laboratory Waste Disposal (Chemistry) compiled by: Chemistry Department Sustainability Committee Overview- *NEW* Glove recycling Hard plastic and transparent glass chemical containers Amber glass chemical containers Uncontaminated soft plastics Styrofoam You can use the template SOP Waste Segregation and Disposal provided in the right-hand column as example. Regardless of which activities a generator decides to conduct in-house, it is imperative that well-trained, qualified staff be available to conduct the waste management activities. Bucket shall be a wash Copyright 2023 National Academy of Sciences. Make an inventory of the types of waste produced by your laboratory. Comments shall be published after review. Southern Africa faces uptick in COVID-19 cases. Nevertheless, there are still justifiable and legal reasons to carry out such operations in the laboratory when hazards can be minimized safely. See below to determine if waste is hazardous or not. Each container listed on the form must have a proper shipping name, hazard class, and EPA number. For example, NIHs low-level mixed waste (LLMW) minimization program demonstrated that a significant amount of mixed waste currently being generated can be reduced or eliminated. For regulatory purposes, the facility needs to keep records for on-site activities that include. Many state and local regulations restrict or prohibit the disposal of waste in municipal landfills or sewer systems, and so it is wise to check the rules and requirements of the local solid waste management authority and develop a list of materials that can be disposed of safely and legally in the normal trash. EPA defines chemical hazardous waste under the Resource Conservation and Recovery Act of 1978 (RCRA 40 CFR Parts 260-272). For biological waste, waste minimization can be accomplished best through careful source separation of biological waste from other waste streams. Corrosivity. If the test strips are not available in the laboratory, the following test can be performed. For example, arsenic wastes are D004. Use hand gloves, safety goggles and mask while disposal of hazardous chemicals. ( Non-infectious waste must be processed according to normal procedures. The determination of whether a waste is regulated as hazardous is usually made either by the institutions EHS staff or by employees of the waste disposal firm. Furthermore, satellite accumulation time must be consistent with the stability of the material. SOP for Disposal of Expired Chemicals, Reagents - Pharmaguideline The overriding principle governing the prudent handling of laboratory waste is that no activity should begin unless a plan for the disposal of nonhazardous and hazardous waste has been formulated. Categorization procedures must account for the common laboratory waste management practices of placing small containers of waste chemicals into a larger overpack drum, and combining of many solvents and solutes into a single drum of flammable liquids. To be drained carefully with continuous flow of water into sink. Print the SOP and put it in a new folder entitled "Biosafety Manual". On-site decay-in-storage of LLW is very efficient and minimizes handling and transportation risks. The acidic wash and rinse solutions contain radio-nuclides and lead and must be handled as mixed waste. Maintain Batch wise destruction record with signature of chemist in Finished Product Register. Appropriate Personal Protective Equipment (PPE) shall be used at the time of disposal. Some states do allow small-scale treatment of waste, but many do not. The second strategic tier is to reuse unwanted material, redistribute surplus chemicals, and reduce hazards. Please share these SOPs to all concern pharma people for their development. Chemical constituents can be separated from mixed waste by using supercritical fluid extraction (e.g., carbon dioxide), which is now available commercially. shall be removed from the 5.0 % Dettol/teepol solution and thoroughly rinse with WHO reiterates its support for the revitalization of Primary Health Care in Angola. If you would like to change your settings or withdraw consent at any time, the link to do so is in our privacy policy accessible from our home page.. The following tests detect, most (but not all) peroxy compounds, including all hydroperoxides. High-performance liquid chromatography, used to purify radiolabeled proteins and lipids, can generate a waste radioactive solution of acetonitrile, water, methanol, acetic acid, and often a small amount of dimethylformamide. This decision tree shows the sequence of tests to be performed to determine the appropriate hazard category of an unknown chemical. For example, the substitution of nonignitable liquid scintillation fluid (LSF) for toluene-based LSF reduces a chemicalradioactive waste to a radioactive waste. The use of plastic (e.g., polyethylene jerry cans) or metal (galvanized or stainless steel) safety containers for the collection of liquid waste is strongly encouraged. Before beginning a detailed discussion of the handling of waste once it has been generated, it is important to understand the definition of waste, how it is characterized, and the regulations that govern it. Depending on the requirements of the disposal facility, adjustment of the pH of aqueous waste may be required. 609-258-4605, Environmental Health and Safety Issue 003: Changed SOP to new format o Changed font from Arial to Calibri o Changed layout including headings format Added Storage and Disposal of Waste by Instrument Section 4. quantities and identification of waste generated and shipped; documentation of analyses of unknown materials if required; manifests for waste shipping as well as verification of disposal; inspection records, training records, contingency plans; and. For instance, flammable liquids have a D001 hazardous waste ID number; corrosives are D002, and reactive wastes are D003. Table 8.1 describes the tasks involved in initiating off-site disposal and provides recommendations for what should be done in-house by staff and what should be contracted to professional service companies. It is important to keep toxic metals (e.g., lead, mercury) out of the incinerable waste so that the ash is not chemically hazardous according to the TCLP test. Glass bottles are impervious to most chemicals but present a breakage hazard, and narrow-neck bottles are difficult to empty. Laboratory Hazardous Waste Management and Disposal Manual Table of Contents 1. Water reactivity. Records are needed both to meet regulatory requirements and to help monitor the success of the hazardous waste management program. Moreover, different categories may not always be compatible with each other and mixing may lead to accidents. For radioactive putrescible waste, off-site disposal requires special packaging, storage, and transport considerations. Use nonradioactive substitutes such as scintillation proximity assays for phosphorus-32 (32P) or sulfur-35 (35S) sequencing studies or 3H cation assays, and enhanced chemiluminescence as a substitute for 32P and 35S DNA probe labeling and Southern blot analysis. There are several acceptable information sources for waste characterization, including the identity of the source or raw materials, in-laboratory test procedures (such as those described below), and analysis by an environmental laboratory. To prevent generation of radioactive aerosols, destruction of needles by grinding or a similar means is not recommended. Most nonhalogenated organic liquids are less dense than water. Consider substituting a less-hazardous constituent for either the chemical or the radioactive source of the mixed waste. USNRC requires that all such sewer-disposable waste be dispersible within the liquid effluent. 2. They are responsible for evaluating hazards, providing information necessary to make an accurate waste determination, and assisting in the evaluation of appropriate strategies for management, minimization, and disposal. Laboratory chemical hoods appropriate for such operations should be designed to trap any radioactive effluent. Development of a yellow-brown color indicates an oxidizer. Do you want to take a quick tour of the OpenBook's features? Carefully add a small quantity of the unknown to a few milliliters of water. Such efforts are most successful when scientists and EHS staff work together to evaluate laboratory processes. We and our partners use cookies to Store and/or access information on a device. Autoclaving waste containing flammable liquids may result in a fire or explosion. Glassware contaminated with a hazardous chemical should be placed in the container designated for solid chemical waste. for 1 hour. Federal regulations regarding transport and incineration may apply to the off-site management of nonlaboratory biohazardous waste, such as waste generated in medical or health care settings. hmO0_nPDBHPXx"A~wW$mn6a|>_[34)_k9Zh&DdQLq!R=)NN|^!7-$~ii}QS&I~7gJ&>C9y~|. (See Chapter 9, for more details. I like to fullfill the need of curious people. In many cases, vehicles must be placarded. Surface contamination from radioactively contaminated lead can be removed by dipping the contaminated lead into a solution of 1 M hydrochloric acid. In general, existing commercial disposal facilities are in business to manage mixed waste from the nuclear power industry, not waste from laboratories. While the first two tiers are the preferred ways of managing chemical waste, the third strategic tier also provides safety and environmental benefits (see Section 8.B.6). Additional exposure precautions may be required. CHEMICAL WASTE DISPOSAL 1.0 HAZARDOUS WASTE DISPOSAL AT THE UNIVERSITY OF NOTRE DAME You can help ease the problem of chemical waste disposal. PROCEDURE: All used chemicals, Reagents and analyzed products shall be stored in HDPE containers after making slurry with suitable solvents. (Regulatory issues, specifically RCRA, are discussed further in Chapter 11, section 11.E.1. Superfund, the federal environmental policy initiative officially known as the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA), provides EPA with broad authority to initiate the cleanup of hazardous waste sites. To ensure compliance, be sure to check local and state regulations that may apply, and seek a legal review if any clarification is needed. Understand that the following test procedures are only to provide additional information, and do not meet EPA regulatory requirements for waste analysis. (See Chapter 4, section 4.D.3.2, for information on the formation and identification of peroxides. Please don't spam. SOP for Disposal of Expired Chemicals, Reagents, Solvents and Micro-biological Medium. If the waste is not a common chemical with known characteristics, enough information about it must be supplied to satisfy the regulatory, requirements and to ensure that it can be handled and disposed of safely. Separate containers of incompatible materials physically or otherwise stored in a protective manner. For this allowance, the container must be kept closed except when adding or removing waste, and all standard time limits for accumulation and container management apply. Final preparations for off-site disposal usually occur at the central accumulation area. Although some laboratories distill waste solvents for reuse, these strategies are most commonly accomplished by sending the waste to a commercial recycling or reclamation facility or to a fuel blender. Media shall be remove Radioac-. Close the neck of the bag and twist it. These liquids may also be used as a fuel source for rotary kilns. Because the hazards of the materials being tested are unknown, the use of proper personal protection and safety devices such as chemical hoods and shields is imperative. Neutralise waste liquids like Acids, alkali, Solutions, slurry, KF titration waste etc. All such type of waste to be packed in bag and then it is to be handed over to ETP for further action. these definitions are unique, especially the definition of waste having the characteristic of toxicity. Transporters typically complete the manifest, but the shipper (generator) is legally responsible for its accuracy and completeness. Infectious waste should be stored separately in a secure area. taking appropriate safety precautions for handling the unknown, including the use of personal protection devices, remove a small sample for use in the following tests. Many low-level radiation materials can then be allowed to decay to a safe level, following which simple disposal is allowable. place clearly labeled containers for each waste category in each laboratory room. Testing is not necessarily required, and in most cases trained laboratory personnel are able to provide sufficient information about the waste to categorize it by general hazard categories. These sites have the capacity to manage LSF, halogenated organics, and other organic waste. If the waste supports the growth of an infectious agent that it contains, storage should be in a freezer to prevent the wastes infectious load from increasing. The selection of which activities to perform in-house and which services to handle through firms that specialize in waste disposal is dependent on the number, qualifications, and availability of in-house staff, organizational philosophy, and budgetary constraints. Waste disposal - disposal of laboratory wastes (guidance) Procedures for Autoclaving Waste ***NOTE: THIS SOP IS APPLICABLE TO BSL1 LAB WASTE ONLY! Yet, if permitted by the sewer facility, it is often reasonable to consider disposal of some chemical waste materials in the sanitary sewer. In-Lab Disposal Methods: Waste Management Guide: Waste Management In most cases, storage of this waste is limited to 90 days and must be managed at an EPA-permitted chemical waste facility. There are federal and state regulations that require all generators of chemical waste receive training and follow proper waste management and disposal procedures. 395 Pine Tree Road, Suite 210 Ithaca, NY 14850. List of Documents needed to register a laboratory to Pollution Control Board. Although expensive, some waste disposal firms offer on-site services to categorize unknown laboratory waste to prepare it for shipment to their treatment facility. Reduce the volume of dry waste by compaction of contaminated waste gloves, absorbent pads, and glassware. Empty containers are no longer subject to federal regulation. Decay-in-storage is usually limited to half-lives of less than 65 days. I.e. Laboratory decay-in-storage space can provide safe storage for low-risk mixed wastes. Therefore it is important that the laboratory adheres to strict procedures regarding waste disposal and processing. Solutions containing flammable or hazardous waste, even if water-miscible, are almost never allowed, and water-immiscible substances must never be put down the drain. When the identity of the material is not known, simple in-laboratory test procedures can be carried out to determine the hazard class into which the material should be categorized. Note that these strips must be air dried until the solvent evaporates and exposed to moisture for proper operation. Once a decision has been reached to hire a waste disposal firm for such services, it is important to know how to select, monitor, and work with such firms. Determine options for managing the multihazardous waste. For example, if safe facilities are available on-site, hold short-half-life radioactive waste for decay before managing it as a chemical or biological waste. Based on feedback from you, our users, we've made some improvements that make it easier than ever to read thousands of publications on our website. Because of the great variety of laboratory mixed waste, it is often difficult to find a facility that can manage both the radioactive and the chemical hazards of the waste. This revised edition has an expanded chapter on chemical management and delves into new areas, such as nanotechnology, laboratory security, and emergency planning. The small-scale treatment, hazard reduction procedures, and deactivation of products (and byproducts) as part of the experiment plan make sense for certain wastes and certain situations at the level of the actual generator, the trained laboratory personnel. Note that there is an optional alternative federal standard for the accumulation of waste within laboratories of colleges, universities, teaching hospitals, and certain nonprofit research facilities associated with colleges or universities. The reactivity classification includes substances that are unstable, react violently with water, detonate if exposed to some initiating source, or produce toxic gases. Economic factors alone should not govern choices, because the long-term consequences can be significant. This array of waste constituent hazards makes the management of multihazardous wastes difficult and complex. For transport on public roads, federal regulations require laboratory waste to be contained in approved packages, such as drums approved by DOT. Drums of commingled waste usually require an analysis or listing of their contents. There are more waste management options for nonregulated waste, especially with regard to hazard reduction procedures. In some cases, chemical disinfection and treatment can be combined to destroy both infectious agents and the drug. Place a small sample of the material (<5 mL) in an aluminum test tray. Safety must be carefully considered before beginning any work. The management of multihazardous waste is particularly challenging for research laboratories where there are frequent changes in protocols, procedures, materials, and waste generating processes.
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sop for waste disposal in laboratory
