We recommend using a Uninterruptible Power Supply (UPS) for the Microlab STAR. Microlab STAR User Manual 610766/02 Page 1 of 225 for User Software 3.0 including Options and Accessories HAMILTON Bonaduz AG. Save method B. The precision of the balance used is less than 1/6 that of the specified pipetting precision (for one channel). liquid level, to prevent the aspiration of air. Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. To reattach the container, simply slot it back into its original position. Hamilton Microlab STAR Service Manual.pdf Microlab STAR - User manual.pdf. For conductive liquids, capacitive LLD should normally be used. Run execution (for running methods on the instrument). For volume verification in the field, HAMILTON will supply a verification tool, based on gravimetrical measurements (the gold standard of volume verification). This is illustrated below with the deck editor as master: Alternatively, the user may start with the method editor as the master editor (if there is no shortcut to the method editor, create it manually. CO-RE tips come in 3 sizes: low-volume, standard, and high-volume (approximately 10, 300, and 1000 l respectively). Compact model provides smooth interplay between software, mechatronics and intuitive user interface. The Hamilton Heater Shaker (HHS) automates the heating and shaking of ANSI/SLAS footprint microplates. The waste level sensor has to be connected with the controller. the tips are RNAseand DNAse-free. During operation, the Microlab STAR should be shielded from direct sunlight and intense artificial light. For this purpose a Liquid Editor comes with the Microlab STAR User Software. In addition, the iSWAP is able to load and unload plates to and from a plate stacker on the left side of the instrument outside the working area (with some restrictions, this can also be done on the right side). 3.fresh needles are picked up from the third module for pipetting, used, and then placed back into the same module. However, the STAR control program (Venus) can be diffi-cult to learn and operate. The waste is collected in the closed-system waste container with liquid level monitoring to prevent overflows. When using Microlab STAR, Good Laboratory Practices (GLP) should be observed. In both cases, a master or central editor is selected, which can either be the method or the deck layout editor. This is called defining a deck layout. Normally, the User Software is used to control the instrument. If sampling aggressive liquids, use filter tips. by aspirating 10 l and dispensing only 9 l. the manner of pipetting (e.g. If a clot is present, it bridges the distance and the signal will remain, resulting in an error message. Connect the wash station only to an earthed outlet. Tips or reusable needles of three sizes on the same head in the same run. This online help will answer any question you may have about details of the Microlab STAR User Software. By continuing to browse the site you are agreeing to our use of cookies. The procedures contained within this manual have been tested by the manufacturer and are deemed to be fully functional. After the life cycle of the instrument has terminated, the Microlab STAR may be shipped to the original manufacturer or retailer. HSL can be accessed directly using the HSL Method Editor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his site uses cookies. It is a WindowsTM-based, menu-driven interface allowing the user to define deck layouts and methods, and then to run the Microlab STAR. Loading Document. Pipetting mode and liquid class represent two independent sets of information which both have to be specified. PDF 1000l channels pipetting specifications Different tabs are visible: Select your instrument configuration. Reviews There are no reviews yet. 10 aliquots of 20 l of a ZDWHU OLNH OLTXLG with the ML-STAR, aspirate 240 l and dispense 20 l directly back into the. The needle wash station is a device for the washing and drying of steel needles parallel to the pipetting process. Guard against software viruses. PDF olab Micr STAR PDF for User Software 3 - Internet Archive Jet Dispense Part Volume for dispensing only a part of the liquid in the tip in a jet, i.e. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. The Hamilton Microlab STAR and the Hudson SOLO robots both use this graphical approach to make their systems approachable to biologists [44, 48]. If the Microlab STAR becomes contaminated with biohazardous material, it should be cleaned in accordance with the maintenance procedures given in the section Maintenance (3.7). Be the first one to, manual_Hamilton_Microlab_STAR_User_Manual, Advanced embedding details, examples, and help, Terms of Service (last updated 12/31/2014). without touching a surface, leaving a residual volume in the tip. This height must be chosen to be permanently below the. Simple Aspiration, for all standard cases, Consecutive Aspiration for aspiration with a tip that has already aspirated liquid, and. MICROLAB STAR Line Operator's Manual - Hamilton Robotics No problems with corroded tubing, pumps, etc. Hamilton Microlab STAR - User manual.pdf | PDF | MedWrench This window is equipped with a magnetic switch and it is locked during a run. The step selection finally allows you to exclude steps such as the move commands from the method editor. This sample tracker is used to generate a Microlab AT-like barcode file using a special filter tool. The valve at the bottom automatically closes, sealing the container. Ensure that the instrument is correctly earthed when connected to the power supply. If you make changes within one of the slave editors, the master editor is automatically updated once you quit the slave editor. web pages The design specifications mentioned above are valid under the following conditions, obtained for measurements at HAMILTON Bonaduz: Gravimetric testing at Hamilton. Depending on the pipetting mode chosen, only a subset of the parameters of the liquid class is active. For the drying of needles an additional parameter is used: -The GUDLQLQJ WLPH gives the length of time allotted for draining the liquid from the needles. Ensure that the the needle wash station is correctly earthed when connected to the power supply. The units can be reprograamed to aliquot other biological samples as well. Keep your head and hands away from the work surface of the Microlab STAR when it is in operation the pipetting arm and channels move fast and it is possible to sustain an injury. One blister pack contains 5 racks of 96 tips, giving a total of 480 tips. The mains plug is on the left hand side of the instrument towards the rear (see picture 1 below). The picture on the left provides a birds-eye view of the vacuum box, showing the conditioning chamber on the right and the elution chamber on the left, which is covered in this picture by the carriage. Given that structure, a system with different HAMILTON instruments (even non-HAMILTON instruments, suitably adapted) can be controlled and operated in the same manner, in the same environment and at the same time. Options cannot be added later, whereas accessories can (see Section 3.3 Accessories below). Such a product has to be properly decontaminated and marked. Same commonly accepted pipetting principle as for hand pipettes. The trueness and precision specifications given for the STAR Line refer to an instrument with 8 x 1000l pipetting channels. Start the Method Editor as the (first opened) master editor. Always dispense to a surface. Note in particular the file contents of the following directories: /RJILOHV: Com Traces (Port Trace), Tip Counter File (TipCount.xls) 0HWKRGV: Methods (*.med, *.hsl), Deck Layouts (*.lay), Method Traces (*.trc), /LEUDU\: HSL libraries of commonly-used additional functions. The instrument-specific steps (e.g. The aspiration monitoring can be switched on and off for each individual aspiration step of a method using the appropriate commands (see section 2.4). An additional partition space is provided for the tip waste container. Microlab 600 Basic Manual - Hamilton Company The 'Hamilton Deck Layout Editor is started by double-clicking the shortcut icon displayed on the desktop of your PC. Note that the availability of accessories is subject to change. Jet Dispense Part Volume for dispensing only a part of the liquid in the tip in a jet, i.e. Microlab STAR Liquid Handling Systems by HAMILTON Robotics Manufacturer HAMILTON Robotics | Available Worldwide 4.3 / 5.0 | 75 reviews | Write your own review Trust in the industry-leading Microlab STAR automated liquid handling platform for lasting confidence in results. The naming of carriers follows a systematic nomenclature: ; < = $QQ. Dispensing of the liquid may now occur in two different modes: to the liquid surface or in a jet. Washing starts. ;: stands for the type of labware, placed on the carriers, e.g., TIP (= tips), 1XPEHU: number of items placed on the carrier (plates or tips), 0': medium density (96or 384-well microplates), $QQ identifies the part number revision (e.g. Deionized water with 0.1 % NaCl, 0.01% Tween, 1R ZDUUDQW\ FDQ EH JLYHQ WKDW WKH DERYH VSHFLILFDWLRQV IRU WUXHQHVV DQG SUHFLVLRQ DUH PHW ZLWK DQ\ RWKHU OLTXLG RU HQYLURQPHQW WKDQ WKH RQHV VSHFLILHG, 2SWLFDO WHVW PHWKRGV VXFK DV IOXRUHVFHQFH RU DEVRUEDQFH SODWH UHDGLQJ WHQG WR KDYH LQWHUQDO VFDWWHUV LQ WKH UDQJH RI DERXW, 7KH RSHUDWLQJ WHPSHUDWXUH UDQJH IRU WKH 0LFURODE 67$5 LV IURP WR & ZLWK D UHODWLYH KXPLGLW\ RI ZLWK QR FRQGHQVDWLRQ. Avoid touching the discarded tips in the waste container. For non-conductive liquids, or in case of an insufficient electrical coupling between container bottom and carrier, pressure LLD should be used. The fuses for the instrument are situated in the mains power switch (see picture 2 below). For each variant there are high-, standardand low-volume options. an analyte recovery experiment was conducted for comparison on a manual platform against the Hamilton Microlab STAR to prove robustness of the validated method on an automated platform. Page 63 and 64: MICROLAB STAR Line Operator's . PLT-CAR-L5MD), whereas carriers with portrait orientation for microplates and tips (e.g. Actively scan device characteristics for identification. It contains a loading head that, shunts carriers onto and off the Microlab STAR, and. To avoid any loss of data, we recommend a UPS (uninterruptable power supply) for the PC. Currently, a mixing of high and low volume channels on one and the same instrument is not feasible. Sample methods for typical applications guide you through the programming. The Microlab STAR is equipped with a pipetting arm typically containing 8 pipetting channels which work independently. According to the different dependencies listed above, liquid classes have attributes related to their intended use: tip type, liquid name, and dispense mode. without touching a surface, leaving a residual volume in the tip. The method editor and the run screen can be started from the deck layout editor by clicking the appropriate buttons located in the leftmost frame. Hamilton - Microlab STAR Manuals and Documents | Medical The pump station has two fuses for the power supply which can be accessed by opening the cover above the mains plug. The operator of the Microlab STAR must have attended an appropriate training course. Microlab 600 Basic Manual Hardware Installation and Basic Operation 1 WELCOME Welcome Welcome to the World of Hamilton Precision Instruments Congratulations on your purchase of a Hamilton Microlab 600 instrument. -The VRDN WLPH is the length of time wash liquid is held within the wash chamber. The system setup allows the user to choose the number of sample plates (up to five) and the number of samples per plate (up to 96). Otherwise local disposal regulations are to be observed. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. $OZD\V NHHS WKH LQVWUXPHQW YROWDJH VHOHFWRU DW 9, 3ODFH WKH DSSURSULDWH IXVH LQ WKH PDLQV SRZHU VZLWFK EHIRUH VZLWFKLQJ RQ WKH LQVWUXPHQW. Close the Deck Layout Editor. One Controller can control up to two vacuum boxes. Steel needles may be picked up directly from the wash station, or from a separate needle rack, which typically is a tip rack on a tip rack carrier with needles instead of tips. The deck layout is defined within the Deck Layout Editor, whereas the aspiration and dispense steps are defined using the method editor. Analyzing the shape of the p(t) curve, the system can distinguish the following situations: -Air is aspirated into the tip (because, for example, the cup has not been filled properly). Analysis, Biological Data Single Steps . 8VLQJ /LEUDU\ )XQFWLRQV WKH 0/ 67$5 /LEUDU\. values). The IORZ UDWH is given in millilitres per second for both wash liquids. The labware is placed on special carriers which are loaded onto the Microlab STAR. Any departure from the procedures given here could lead to erroneous results or malfunction. The Microlab STAR User Software allows an Q Q relation between deck layouts and methods. Utilizing an Automated Platform to Streamline Workflow Processes This can be provided by STARs /LTXLG /HYHO 'HWHFWLRQ (LLD) feature, based. Washing starts. so specified) according to the volume aspirated. The software integrates seamlessly with MICROLAB Vector. The pipetting arm can move in an X direction, whereas each pipetting channel can move relatively independently both in a Y and a Z direction. Plug the mains cables for the computer and the instrument into the same electrical outlet. The customer need only ensure that a suitable control PC is available for installation of the ML STAR User Software. To empty the waste liquid container or to refill the wash solutions, lift the container by the handle. The sample tracker, which generates a database of liquid transfers in the background can be switched on here, too. These carriers hold reagent containers, such as tubes, microtiter plates, or other kinds of labware. HAMILTON has optimized the standard liquid classes with great care to assure the best pipetting accuracy. Retailer Reg: 2019--2018 | The pipetting channels have a set travelling height of 245 mm above the origin, or 145 mm between the tip of the disposable tip and the deck of the instrument. The VRDN WLPH is the length of time wash liquid is held within the wash chamber. For each of the two wash solutions (or wash and rinse solution), a set of parameters can be specified: -The ULQVH WLPH is the length of time liquid flows through the wash chamber. To ensure that the specified accuracy is achieved, volumes below 20 l should always be GLVSHQVHG WR D OLTXLG VXUIDFH. ([DPSOH PLT-CAR-L5MD-A00 is a carrier for 5 medium density (96or 384-well) microplates in landscape orientation. The Microlab STAR requires a recent model of PC including a mouse, CD-ROM drive, high resolution monitor and graphics (SVGA or better), 1-2 serial ports for a USB, about 1 GB HDD and 128 MB RAM, and Windows 2000 (service pack 1 or better) as the operating system. The process is repeated again. You should read through the entire manual before beginning to operate your instrument. There are no reviews yet. This is done with the tips still in the air. A carrier must always be identified (e.g in deck layouts and methods) by the unique descriptor with which it is tagged. Additional flexibility allows userdefined volumes . A user will typically wish to carry out low, medium, or high volume contamination-free pipetting with disposable tips or with steel needles. This is illustrated below. This could result in incorrect test data. container. For HMH96 microtitre plate handling, the instrumentation setup requires a CO-RE 96 probe head, and for serial dilutions, an 8-channel array of 1000 L channel pipettes. This manual is for users of the Hamilton MICROLAB STAR IVD / STARlet IVD pipettor component of the cobas s 201 system. STAR stands for Sequential Transfer and Aliquoting Robot. As the controller requires 24 volts, a power supply with 24 volts is delivered together with the controller. The run simulator creates a method trace file with pipetting pattern and other useful information. For aspiration, three modes are possible: Simple Aspiration, for all standard cases, Consecutive Aspiration for aspiration with a tip that has already aspirated liquid, and. In some cases, one may wish to define special labware items or sequences (pipetting patterns - see below) using the labware editor and the sequence editor. Page 73 and 74: MICROLAB STAR Line . The manual describes both the hardware and software of the Microlab STAR to the extent that a user needs to know them in order to operate the instrument. The Microlab STAR User Software allows the user to program and run different methods for aspirating and dispensing liquids. Select Save As to save method A under the new name method B. from less than 1 l to 300 l (for the use of low volume tips = 10 l, and standard tips = 300 l), or. The installation generates the following default directory structure, if not requested otherwise. A set of default parameters is given for the wash process within the relevant dialog boxes of the User Software. Automated Liquid Handling Platforms Customers can order one of two complete variants of the wash station: regular, suitable for most uses, and chemical-resistant, specially designed to meet the needs of those working with aggressive chemicals. Multiple units may be integrated and connected via a control box for higher throughput applications. Hamilton designed carriers hold your labware and provide flexibility to configure the deck tailored specifically for your assays. To enable parallel pipetting with an 8-channel Microlab STAR, the following cycle of steps takes place: 1.fresh needles are picked up from the first module for pipetting, used, and then placed back into the same module. HAMILTON or the authorised HAMILTON representative will repair or replace, at its option and free of charge, any product that under proper and normal use proves to be defective during the warranty period. PDF HSPA-A universal graphical user interface for the Hamilton Microlab There is also a push-button switch that allows the user to manually operate the carriage. These methods are then either executed or simulated. The Microlab STAR User Software allows the user to program and run different methods for aspirating and dispensing liquids. Use only manufacturers original installation CD-ROM sets for the operating system, and the original HAMILTON software. Revolutionize the work in your lab by replacing manual pipetting with the efficiency and accuracy of the Microlab Prep. F ?EDUFRGHV In addition, this directory is used by the sample tracker to store Microlab AT-like barcode and register files. In this case, aspiration monitoring is deactivated and the tip will follow the falling liquid level (if specified) to the bottom of the container, staying there for the rest of the aspiration. graphical and the HSL method editor. The TCC will be installed by a trained Hamilton service engineer. The Hamilton Microlab consists of highly customisable liquid handling and vessel handling devices, with plates or tips are mounted in carriages. Run simulation is a tool to test whether a method has been programmed correctly. Personalised ads and content, ad and content measurement, audience insights and product development. The pipetting specifications for the Microlab STAR are given in the following table. Pictured above is the right side of the controller with the different connections. The process is repeated again. Filter plates can be placed onto or removed from the system by hand or with the help of a robotic arm (see the section on iSWAP below). The wash station has the width of a normal microplate carrier (6T) and is mounted on the deck of the Microlab STAR. Select the appropriate voltage at the pump station of the needle washer. One wash station consists of three individual 8-fold wash modules IRU WKH VDPH QHHGOH W\SH: High Volume (1000 l), Standard Volume (300 l), or Low Volume (50 l). Always dispense to a surface. MICROLAB STAR Line Operator's Manual - Hamilton Robotics - Yumpu The AVS and its components are to be installed by a Hamilton service engineer. All tubing and electrical connections are routed through the docking station at the rear of the instrument.
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hamilton microlab star user manual
